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pRc/CMV HA TrkC
(Plasmid #39979)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39979 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRC/CMV AC7 vector, a derivative of the pRC/CMV vector
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TrkC
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2500
  • Mutation
    endogenous signal sequence was replaced with BM40 and HA tag
  • GenBank ID
    NM_001270656.1
  • Entrez Gene
    Ntrk3 (a.k.a. trkC)
  • Promoter CMV
  • Tags / Fusion Proteins
    • BM40 signal sequence (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (PmeI added) (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide

Please note that the endogenous signal sequence of TrkC was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to translated sequence for GenBank ID NM_001270656.1, the TrkC protein sequence starts at amino acid #32

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/CMV HA TrkC was a gift from Yves-Alain Barde (Addgene plasmid # 39979 ; http://n2t.net/addgene:39979 ; RRID:Addgene_39979)
  • For your References section:

    Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. Bibel M, Hoppe E, Barde YA. EMBO J. 1999 Feb 1;18(3):616-22. 10.1093/emboj/18.3.616 PubMed 9927421