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(Plasmid #39977)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 39977 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pRC/CMV AC7 vector, a derivative of the pRC/CMV vector
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Alt name
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
  • Mutation
    endogenous signal sequence was replaced with BM40 and HA tag
  • GenBank ID
  • Entrez Gene
    Ntrk1 (a.k.a. Trk)
  • Promoter CMV
  • Tags / Fusion Proteins
    • BM40 signal sequence (N terminal on insert)
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (pmeI added) (unknown if destroyed)
  • 3′ cloning site Not I (internal NotI site was silently mutated) (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

cloned in the pRC/CMV AC7 vector, a derivative of the pRC/CMV vector (Invitrogen), which contains the BM40 signal peptide

Please note that the endogenous signal sequence of TrkA was replaced by the BM40 signal peptide sequence (MRAWIFFLLCLAGRALAAPLA). When compared to GenBank ID NP_067600.1, the TrkA protein sequence starts at amino acid #30

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/CMV HA TrkA was a gift from Yves-Alain Barde (Addgene plasmid # 39977 ; ; RRID:Addgene_39977)
  • For your References section:

    Biochemical and functional interactions between the neurotrophin receptors trk and p75NTR. Bibel M, Hoppe E, Barde YA. EMBO J. 1999 Feb 1;18(3):616-22. 10.1093/emboj/18.3.616 PubMed 9927421