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pFM032
(Plasmid #40076)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40076 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIC26
  • Backbone manufacturer
    Cheeseman lab
  • Backbone size (bp) 16397
  • Modifications to backbone
    GFP replaced with Dendra2 PAFP (sequence optimized for worm expression; three synthetic introns inserted)
  • Vector type
    Worm Expression
  • Promoter pie-1
  • Selectable markers
    unc-119
  • Tags / Fusion Proteins
    • Dendra2 PAFP (N terminal on backbone)
    • TEV protease cleavage site (N terminal on backbone)
    • S-peptide (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Cloning Information

  • Cloning method Restriction Enzyme

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternate plasmid name: pIC26 + wormanized Dendra:speI

Gene of interest should be cloned into the SpeI restriction site to generate Dendra2 FPAP2 N-terminal fusion proteins.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFM032 was a gift from Geraldine Seydoux (Addgene plasmid # 40076 ; http://n2t.net/addgene:40076 ; RRID:Addgene_40076)
  • For your References section:

    Microtubules induce self-organization of polarized PAR domains in Caenorhabditis elegans zygotes. Motegi F, Zonies S, Hao Y, Cuenca AA, Griffin E, Seydoux G. Nat Cell Biol. 2011 Oct 9;13(11):1361-7. doi: 10.1038/ncb2354. 10.1038/ncb2354 PubMed 21983565