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(Plasmid #40084)


Item Catalog # Description Quantity Price (USD)
Plasmid 40084 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4768
  • Total vector size (bp) 6879
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamicin, 10 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    gfpmut 3.1
  • Species
  • Insert Size (bp)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
  • Species
    Sinorhizobium meliloti
  • Insert Size (bp)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    tau promoter
  • Species
    Sinorhizobium meliloti
  • Insert Size (bp)

Cloning Information for Gene/Insert 3

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

GenBank accession number: JQ895027

pLMB509 contains GFPmut3.1 reporter gene downstream of the inducible promoter tauAp and a ribosomal binding site (RBS), followed by a six-His tag and a transcriptional stop codon. NdeI restriction sites flank the reporter gene, enabling the easy removal of gfpmut3.1 and subsequent replacement with the desired gene in frame.

This plasmid is compatible with BD In-Fusion cloning (Clontech) for the cloning of genes containing NdeI restriction sites

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLMB509 was a gift from Philip Poole (Addgene plasmid # 40084 ; ; RRID:Addgene_40084)
  • For your References section:

    Regulatable vectors for environmental gene expression in alphaproteobacteria. Tett AJ, Rudder SJ, Bourdes A, Karunakaran R, Poole PS. Appl Environ Microbiol. 2012 Oct;78(19):7137-40. Epub 2012 Jul 20. 10.1128/AEM.01188-12 PubMed 22820336