pEQ111m
(Plasmid #40190)

• Depositing Lab: Paul Modrich
• Publication: Wright et al J Biol Chem. 1989 Jul 15;264(20):11816-21.

Backbone

• Vector backbone: pSCC3
• Backbone manufacturer: n/a
• Backbone size w/o insert (bp): 3000
• Total vector size (bp): 3200
• Modifications to backbone: none
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): JC4588 lambda+
• Growth instructions: Induce expression by transfer to 37˚C.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EcoRI endonuclease & EcoRI methylase
• Species: E. coli
• Insert Size (bp): 2210
• Mutation: changed Glutamic Acid 111 to Glutamine
• GenBank ID: J01675.1
• Promoter: Lambda PL

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (unknown if destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: NNNNNN
• 3′ sequencing primer: NNNNNN

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pSCC3 plasmid derivation is described in King, K. et al. (1989) J.Biol.Chem. 264, 11807-11815.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.