|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45176||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 3400
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Speciesjelly fish and coral fluorescent proteins
Insert Size (bp)3400
- Cloning method Gateway Cloning
- 5′ sequencing primer ACAAGTTTGTACAAAAAAGCAGGCT
- 3′ sequencing primer ACCACTTTGTACAAGAAAGCTGGGT (Common Sequencing Primers)
Please note that the backbone sequence is from a non Gateway pCAG vector and may be missing a few features. These sequencing mismatches do not affect plasmid function. Please refer to Addgene's restriction digest for accurate representation of the plasmid size.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-Brainbow3.0 was a gift from Joshua Sanes (Addgene plasmid # 45176 ; http://n2t.net/addgene:45176 ; RRID:Addgene_45176)
For your References section:Improved tools for the Brainbow toolbox. Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR. Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 2013 May 5. 10.1038/nmeth.2450 PubMed 23817127