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pCAG-Brainbow3.0 Notes

Addgene Diagnostic Digest for 45176

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pCAG-Brainbow3.0 with the following enzymes:Pme I, SaII-HF, EcoRI-HF and SaII-HF + Pme I double digest.

The digest result suggests that the total plasmid size is ~8kb, rather than the expected 5.7kb and SalI cuts the plasmid twice, rather than once.