Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39993||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
- Total vector size (bp) 5200
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin (50 ug/mL)
Growth Strain(s)JC4588 lambda+
Growth instructionsInduce expression by transfer to 37˚C.
Gene/Insert nameEcoRI endonuclease & EcoRI methylase
Insert Size (bp)2210
- Promoter n/a
/ Fusion Protein
- Cloning method Restriction Enzyme
- 5′ cloning site HpaI (unknown if destroyed)
- 3′ cloning site HpaI (unknown if destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSCC2 was a gift from Paul Modrich (Addgene plasmid # 39993 ; http://n2t.net/addgene:39993 ; RRID:Addgene_39993)
For your References section:Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain. Cheng SC, Kim R, King K, Kim SH, Modrich P. J Biol Chem. 1984 Sep 25;259(18):11571-5. PubMed 6088551