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pSCC2
(Plasmid #39993)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 39993 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pKC30
  • Backbone manufacturer
    n/a
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin (50 ug/mL)
  • Growth Temperature
    30°C
  • Growth Strain(s)
    JC4588 lambda+
  • Growth instructions
    Induce expression by transfer to 37˚C.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EcoRI endonuclease & EcoRI methylase
  • Species
    E. coli
  • Insert Size (bp)
    2210
  • Mutation
    none
  • GenBank ID
    J01675.1
  • Promoter n/a
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HpaI (unknown if destroyed)
  • 3′ cloning site HpaI (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

pKC30 plasmid derivation is described in Nature (1981) 292, 128-132.

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] if you have any questions.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCC2 was a gift from Paul Modrich (Addgene plasmid # 39993 ; http://n2t.net/addgene:39993 ; RRID:Addgene_39993)
  • For your References section:

    Isolation of gram quantities of EcoRI restriction and modification enzymes from an overproducing strain. Cheng SC, Kim R, King K, Kim SH, Modrich P. J Biol Chem. 1984 Sep 25;259(18):11571-5. PubMed 6088551