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Depositing Lab
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Depositing OrganizationUniversity of Pittsburgh (University, School of Medicine, and Cancer Institute)
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40201-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40201-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepcDNA6.V5.HisB
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Backbone manufacturerInvirtogen
- Backbone size w/o insert (bp) 4985
- Total vector size (bp) 5556
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Modifications to backbonepcDNA6.V5.HisB vector was modified with NheI and PmeI restriction digestion enzymes to cut out MCS, V5 and His tag sites. New multiple cloning sites using annealed primer pair were ligated back into the vector. The newly generated cloning site comprises: NheI, KpnI, EcoRV, XhoI and SacII. Codon optimized sT was cloned between EcoRV and XhoI restriction sites.
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMCPyV sT codon optimized
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Alt nameMCV sT
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Alt nameMCV small T antigen
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Alt nameMerkel Cell Polyomavirus small T antigen
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SpeciesVirus
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Insert Size (bp)571
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GenBank IDAEM01096.1
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 40201-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40201-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA6 MCV sTco was a gift from Patrick Moore (Addgene plasmid # 40201 ; http://n2t.net/addgene:40201 ; RRID:Addgene_40201) -
For your References section:
Human Merkel cell polyomavirus small T antigen is an oncoprotein targeting the 4E-BP1 translation regulator. Shuda M, Kwun HJ, Feng H, Chang Y, Moore PS. J Clin Invest. 2011 Sep;121(9):3623-34. doi: 10.1172/JCI46323. Epub 2011 Aug 15. 10.1172/JCI46323 PubMed 21841310
Map uploaded by the depositor.
