Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||28193||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepcDNA6/V5-His B
- Backbone size w/o insert (bp) 5100
Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameMCPyV Truncated Tumor Large T antigen (399 tumor strain)
Alt nameHuman Merkel Cell polyomavirus
SpeciesHuman Merkel Cell polyomavirus
Insert Size (bp)2003
/ Fusion Protein
- V5 (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRV (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA6.MCV.LT339.V5.4 was a gift from Patrick Moore (Addgene plasmid # 28193 ; http://n2t.net/addgene:28193 ; RRID:Addgene_28193)
For your References section:T antigen mutations are a human tumor-specific signature for Merkel cell polyomavirus. Shuda M, Feng H, Kwun HJ, Rosen ST, Gjoerup O, Moore PS, Chang Y. Proc Natl Acad Sci U S A. 2008 Oct 21. 105(42):16272-7. 10.1073/pnas.0806526105 PubMed 18812503
Map uploaded by the depositor.