pcDNA6 MCV LTco S220A
(Plasmid #112193)

• Purpose: Expression of MCPyV LT mutant (Ser 220 to Ala)
• Depositing Lab: Patrick Moore
• Publication: Kwun et al Proc Natl Acad Sci U S A. 2017 May 16;114(20):E4040-E4047. doi: 10.1073/pnas.1703879114. Epub 2017 May 1.

Backbone

• Vector backbone: pcDNA6.V5.HisB
• Backbone manufacturer: Invirtogen
• Backbone size w/o insert (bp): 4985
• Total vector size (bp): 7549
• Modifications to backbone: pcDNA6.V5.HisB vector was modified with NheI and PmeI restriction digestion enzymes to cut out MCS, V5 and His tag sites. New multiple cloning sites using annealed primer pair were ligated back into the vector. The newly generated cloning site comprises: NheI, KpnI, EcoRV, XhoI and SacII. Codon optimized Large T was cloned between EcoRV and XhoI restriction sites.
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MCPyV LT codon optimized
• Alt name: MCV LT
• Alt name: MCV large T antigen
• Alt name: Merkel Cell Polyomavirus large T antigen
• Species: Merkel cell polyomavirus
• Insert Size (bp): 2564
• GenBank ID: AEM01097.1
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGHr

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry