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PurposeMammalian expression of codon-optimized Trex2.
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Depositing Lab
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Depositing OrganizationUniversity of Washington
Located in United States of America -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40210 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40210-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40210-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUC
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Backbone manufacturerIn-house
- Backbone size w/o insert (bp) 4389
- Total vector size (bp) 5100
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Modifications to backboneMultiple cloning site, CMV and T7 promoters
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameThree prime repair exonuclease 2
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Alt nameTrex2
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SpeciesM. musculus (mouse)
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Insert Size (bp)711
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Entrez GeneTrex2
- Promoter CMV and T7
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Nhe1 (not destroyed)
- 3′ cloning site EcoR1 (not destroyed)
- 5′ sequencing primer T7 FP
- 3′ sequencing primer BGH RP
- (Common Sequencing Primers)
Resource Information
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Addgene Notes
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A portion of this plasmid was derived from a plasmid made byWe received the Trex2 ORF-containing construct from the laboratory of Daniel Stetson, University of Washington
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 40210-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40210-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pExodus CMV.Trex2 was a gift from Andrew Scharenberg (Addgene plasmid # 40210 ; http://n2t.net/addgene:40210 ; RRID:Addgene_40210) -
For your References section:
Coupling endonucleases with DNA end-processing enzymes to drive gene disruption. Certo MT, Gwiazda KS, Kuhar R, Sather B, Curinga G, Mandt T, Brault M, Lambert AR, Baxter SK, Jacoby K, Ryu BY, Kiem HP, Gouble A, Paques F, Rawlings DJ, Scharenberg AM. Nat Methods. 2012 Oct;9(10):973-5. doi: 10.1038/nmeth.2177. Epub 2012 Sep 2. 10.1038/nmeth.2177 PubMed 22941364
