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pClneoMyc human ERBIN
(Plasmid #40214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40214 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 40214-D50 50 μg of DNA in Tris buffer $495
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DNA 40214-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCI-neo (modified)
  • Backbone manufacturer
    Promega
  • Modifications to backbone
    pCI-neo vector was modified to include N-terminal myc tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ERBIN
  • Species
    H. sapiens (human)
  • Mutation
    L143S, S274L and P338L mutation in ERBIN compared to NCBI reference NP_001006600.1
  • Entrez Gene
    ERBIN (a.k.a. ERBB2IP, HEL-S-78, LAP2)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The Addgene verified sequence identified the mutations L143S, S274L, and P338L in ERBIN compared to NCBI reference NP_001006600.1. The functional effects of these mutations are unknown, however the plasmid functions as described in the associated publication.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pClneoMyc human ERBIN was a gift from Yutaka Hata (Addgene plasmid # 40214 ; http://n2t.net/addgene:40214 ; RRID:Addgene_40214)

  • For your References section:

    Localization of p0071-interacting proteins, plakophilin-related armadillo-repeat protein-interacting protein (PAPIN) and ERBIN, in epithelial cells. Ohno H, Hirabayashi S, Iizuka T, Ohnishi H, Fujita T, Hata Y. Oncogene. 2002 Oct 10;21(46):7042-9. 10.1038/sj.onc.1205852 PubMed 12370826
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