Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40568||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 40569
Modifications to backboneIRES EGFP of pMIEG replaced with human CD4 cDNA
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Alt nameGATA binding protein 3
SpeciesM. musculus (mouse)
GenBank IDNM_008091.3 NP_032117.1
Entrez GeneGata3 (a.k.a. Gata-3, jal)
/ Fusion Protein
- IRES hCD4 (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer pLXSN_5
- 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa) (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The coding region for murine GATA-3 cDNA was amplified by PCR and cloned into MIEG-hCD4. Please note that the discrepancies between the QC and NCBI sequences should not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Gata3-MIEG-hCD4 was a gift from Alexander Dent & Mark Kaplan (Addgene plasmid # 40568 ; http://n2t.net/addgene:40568 ; RRID:Addgene_40568)
For your References section:PU.1 expression delineates heterogeneity in primary Th2 cells. Chang HC, Zhang S, Thieu VT, Slee RB, Bruns HA, Laribee RN, Klemsz MJ, Kaplan MH. Immunity. 2005 Jun;22(6):693-703. 10.1016/j.immuni.2005.03.016 PubMed 15963784