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(Plasmid #40599)


Item Catalog # Description Quantity Price (USD)
Plasmid 40599 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 4874
  • Total vector size (bp) 6507
  • Vector type
    Yeast Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    mCherry (Red Fluorescent Protein variant) with yeast promoter and terminator sequences
  • Alt name
  • Species
  • Insert Size (bp)
  • Mutation
    mCherry ORF partially codon optimised for yeast expression
  • Promoter GPD (TDH3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site EagI (not destroyed)
  • 5′ sequencing primer M13f
  • 3′ sequencing primer M13r
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The cassette comprising promoter, mCherry v4 ORF and transcription terminator was subcloned from a construct originally generated by Dr Wenfeng Qian and Prof Jianzhi Zhang, University of Michigan. Qian et al. (2012) Balanced codon usage optimizes eukaryotic translational efficiency. PLoS Genetics 8(3):e1002603.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH761-CEN-mCherry_v4 was a gift from Tobias von der Haar (Addgene plasmid # 40599 ; ; RRID:Addgene_40599)
  • For your References section:

    Translation elongation can control translation initiation on eukaryotic mRNAs. Chu D, Kazana E, Bellanger N, Singh T, Tuite MF, von der Haar T. EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.1002/embj.201385651. Epub 2013 Dec 19. 10.1002/embj.201385651 PubMed 24357599