|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40778||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonep15a, AmpR, CamR
- Backbone size w/o insert (bp) 3209
- Total vector size (bp) 4815
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAraC-pBad + coi
Insert Size (bp)1606
Terms and Licenses
For detailed composition information, look up the backbone (BBa_J72110) and insert (BBa_J72094) on the Registry of Standard Biological Parts (partsregistry.org)
2 nt mismatches, T286C and T444C, both in AraC do not effect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:BBa_J72110-BBa_J72094 was a gift from Christopher Anderson (Addgene plasmid # 40778 ; http://n2t.net/addgene:40778 ; RRID:Addgene_40778)
For your References section:Scalable plasmid transfer using engineered P1-based phagemids. Kittleson JT, DeLoache W, Cheng HY, Anderson JC. ACS Synth Biol. 2012 Dec 21;1(12):583-9. doi: 10.1021/sb300054p. Epub 2012 Aug 30. 10.1021/sb300054p PubMed 23656280