pGEX-mTAZ-WW (86)
(Plasmid #40915)

• Depositing Lab: Michael Yaffe
• Publication: Kanai et al EMBO J. 2000 Dec 15. 19(24):6778-91.

Backbone

• Vector backbone: pGEX-4T-2
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TAZ
• Alt name: WWTR1
• Alt name: Transcriptional co-Activator with PDZ-binding motif
• Alt name: WW domain containing transcription regulator 1
• Species: M. musculus (mouse)
• Insert Size (bp): 250
• Mutation: Contains mTAZ 14-3-3 binding site and WW domain (86 amino acids, 79-164)
• GenBank ID: NM_001168281.1 NM_133784.3
• Entrez Gene: Wwtr1 (a.k.a. 2310058J06Rik, 2610021I22Rik, Taz)
• Promoter: Tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pGEX5
• 3′ sequencing primer: pGEX3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Fragments coding for the mTAZ WW domain (amino acids 122-164 and 79-164) were subcloned into pGEX4T2 (Amersham Pharmacia Biotech) for bacterial expression.