pcDNA3 CHICA (Nigg HS342)
(Plasmid #41153)

• Depositing Lab: Erich Nigg
• Publication: Santamaria et al Curr Biol. 2008 May 20;18(10):723-9.

Backbone

• Vector backbone: pcDNA3.1/3x myc-C
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 7260
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CHICA
• Alt name: C20orf129
• Alt name: FAM83D
• Alt name: family with sequence similarity 83, member D
• Species: H. sapiens (human)
• Insert Size (bp): 1760
• Mutation: V11L
• GenBank ID: NM_030919.2 Q9H4H8.3
• Entrez Gene: FAM83D (a.k.a. C20orf129, CHICA, dJ616B8.3)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 3xMyc (N terminal on backbone)
  • PreScission Site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI/NcoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: CHICA cDNA clone from Deutsches Ressourcenzentrum fur Genomforschung (RZPD) (Berlin)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For cloning, the following cDNA was obtained from the “Deutsches Ressourcenzentrum fur Genomforschung” (RZPD): C20Orf129 (IMAGp958M1212). The DNA coding sequence was amplified by PCR using gene-specific primers and cloned into pcDNA3.1/3x myc-C in-frame with a sequence encoding an amino-terminal triple myc-tag.

Addgene's sequencing results identified a V11L mutation in Chica when compared to GenBank accession number Q9H4H8.3