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pFA6a-His3MX6-PGAL1-GFP
(Plasmid #41616)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 41616 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 41616-D50 50 μg of DNA in Tris buffer $495
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DNA 41616-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pFA6a
  • Vector type
    Yeast genomic targeting
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GAL1 promoter
  • Alt name
    GAL1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2651
  • Entrez Gene
    GAL1 (a.k.a. YBR020W)
  • Tags / Fusion Proteins
    • A. gossypii translation elongation factor 1a gene terminator (N terminal on insert)
    • S. kluyveri His3 reverse orientation (N terminal on insert)
    • A. gossypii translation elongation factor 1a gene promoter (N terminal on insert)
    • GFP(S65T) (C terminal on insert)
    • yeast ADH1 terminator (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site PacI (not destroyed)
  • 5′ sequencing primer T7_promoter
  • 3′ sequencing primer Sp6_primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFA6a-His3MX6-PGAL1-GFP was a gift from John Pringle (Addgene plasmid # 41616 ; http://n2t.net/addgene:41616 ; RRID:Addgene_41616)

  • For your References section:

    Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Longtine MS, McKenzie A 3rd, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Yeast. 1998 Jul;14(10):953-61. 10.1002/(SICI)1097-0061(199807)14:10<953::AID-YEA293>3.0.CO;2-U PubMed 9717241
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