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pAAH6
(Plasmid #41830)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41830 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSET7-26b
  • Backbone manufacturer
    Covalys
  • Backbone size (bp) 3724
  • Modifications to backbone
    Contains the gene for the SNAP tag along with the clonNAT resistance cassette from pAG25 The nourseothricin/ClonNAT cassette was removed from pAG25 (Euroscarf) by digestion with NotI and SacI and ligated into those same sites of the pSET7-26b SNAP plasmid from Covalys/New England Biolabs.
  • Vector type
    Bacterial Expression, Yeast Expression ; PCR template
  • Promoter T7,TEF
  • Selectable markers
    clonNAT/nourseothricin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    SNAP tag was purchased from NEB.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAH6 was a gift from Melissa Moore (Addgene plasmid # 41830 ; http://n2t.net/addgene:41830 ; RRID:Addgene_41830)
  • For your References section:

    Ordered and dynamic assembly of single spliceosomes. Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ. Science. 2011 Mar 11;331(6022):1289-95. 10.1126/science.1198830 PubMed 21393538