pAG306-GPD-ccdB chr I
(Plasmid
#41894)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41894 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAG306GPD-ccdB
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Backbone manufacturerSusan Lindquist (Addgene plasmid# 14140)
- Backbone size (bp) 6972
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Modifications to backboneAdded two regions of homology to S. cerevisiae Chromosome I separated by a Not I restriction site. Plasmid can be integrated into Chr I of S. cerevisiae BY background after digestion with Not I.
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Vector typeYeast Expression
- Promoter GPD
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DB3.1
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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A portion of this plasmid was derived from a plasmid made bypAG306GPD-ccdB was obtained from Addgene
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was generated by ligation of a SmaI-digested fusion PCR product that contained two ~500-base-pair regions of chromosome 1 flanking a NotI site into AatII-digested pAG306-GPD-ccdB.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG306-GPD-ccdB chr I was a gift from Dan Gottschling (Addgene plasmid # 41894 ; http://n2t.net/addgene:41894 ; RRID:Addgene_41894) -
For your References section:
An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast. Hughes AL, Gottschling DE. Nature. 2012 Dec 13;492(7428):261-5. doi: 10.1038/nature11654. Epub 2012 Nov 21. 10.1038/nature11654 PubMed 23172144
Map uploaded by the depositor.
