pAO122 (Apr LEU2 MRC1)
(Plasmid
#41923)
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Depositing Lab
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Depositing OrganizationBaylor College of Medicine
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 41923 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 41923-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 41923-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRS415
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 6021
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMediator of the Replication Checkpoint
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Alt nameMRC1
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SpeciesS. cerevisiae (budding yeast)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Xho1 (unknown if destroyed)
- 3′ cloning site Xho1 (unknown if destroyed)
- 5′ sequencing primer M13_forward20_primer
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
There is a D304N in LEU, relative to LEU start aa, this is not thought to effect function.
DNA (Catalog # 41923-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 41923-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAO122 (Apr LEU2 MRC1) was a gift from Stephen Elledge (Addgene plasmid # 41923 ; http://n2t.net/addgene:41923 ; RRID:Addgene_41923) -
For your References section:
Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Osborn AJ, Elledge SJ. Genes Dev. 2003 Jul 15;17(14):1755-67. 10.1101/gad.1098303 PubMed 12865299