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pAO139 (Apr LEU2 mrcl-AQ)
(Plasmid #41924)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 41924 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRS415
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 6021
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mediator of the Replication Checkpoint
  • Alt name
    MRC1
  • Species
    S. cerevisiae (budding yeast)
  • Mutation
    MRC1AQ

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (unknown if destroyed)
  • 3′ cloning site Pac1 (unknown if destroyed)
  • 5′ sequencing primer M13_forward20_primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a D304N mutation in LEU, relative to aa1 in LEU, it is not thought to affect function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAO139 (Apr LEU2 mrcl-AQ) was a gift from Stephen Elledge (Addgene plasmid # 41924 ; http://n2t.net/addgene:41924 ; RRID:Addgene_41924)
  • For your References section:

    Mrc1 is a replication fork component whose phosphorylation in response to DNA replication stress activates Rad53. Osborn AJ, Elledge SJ. Genes Dev. 2003 Jul 15;17(14):1755-67. 10.1101/gad.1098303 PubMed 12865299