PurposeRetroviral expression of DsRed in mammalian cells
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||41943||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 7556
Vector typeMammalian Expression, Retroviral
Selectable markersIRES d4EGFP
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 50 μg/mL
Growth Strain(s)ccdB Survival
Copy numberHigh Copy
- Promoter TRE
- Cloning method Gateway Cloning
- 5′ sequencing primer pLXSN-5 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
4 hour half life EGFP
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MSCV-CMV-DsRed-IRES-d4EGFP was a gift from Stephen Elledge (Addgene plasmid # 41943 ; http://n2t.net/addgene:41943 ; RRID:Addgene_41943)
For your References section:Global protein stability profiling in mammalian cells. Yen HC, Xu Q, Chou DM, Zhao Z, Elledge SJ. Science. 2008 Nov 7;322(5903):918-23. doi: 10.1126/science.1160489. 10.1126/science.1160489 PubMed 18988847