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pEGFP VAMP7 (1-220)
(Plasmid #42316)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42316 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    VAMP7 (1-220)
  • Alt name
    VAMP7
  • Alt name
    vesicle-associated membrane protein 7
  • Alt name
    TI-VAMP/Synaptobrevin-like (Sybl 1)
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_005638.5 NP_005629.1
  • Entrez Gene
    VAMP7 (a.k.a. SYBL1, TI-VAMP, TIVAMP, VAMP-7)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer EGFP-C; CMV-F
  • 3′ sequencing primer SV40-pA-R
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Alternate plasmid name: Full-length TI-VAMP (TIVAMP)

For production of NH2-terminal GFP fusion proteins, the distinct cDNAs were cloned into the pEGFP-C3 vector (CLONTECH Laboratories, Inc.). Full-length TI-VAMP (TIVAMP), NH2-terminal domain-TIVAMP (Nter-TIVAMP, from M to N120), cytosolic domain-TIVAMP (Cyt-TIVAMP, from M to K188), and ΔNH2-terminal domain-TIVAMP (ΔNter-TIVAMP, from M102 to the end), were obtained by PCR using standard procedures and the following sets of oligonucleotides:

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-CTATTTCTTCACACAGCTTGGCCATGT-3′
for TIVAMP;

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-CTTATTCTCAGAGTGATGCTTCAG-CTG-3′
for Nter-TIVAMP;

5′-ATGGCGATTCTTTTTGCTGTTGTTGCC-3′
and
5′-ATCCTACTTGAGGTTCTTCATACACATGGCTC
for Cyt-TIVAMP;

5′-ATGAATAGCGAGTTCTCAAGTGTCTTA-3′
and
5′-CTATTTCTTCACACAGCTTGGCCATGT-3′
for ΔNter-TIVAMP.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP VAMP7 (1-220) was a gift from Thierry Galli (Addgene plasmid # 42316 ; http://n2t.net/addgene:42316 ; RRID:Addgene_42316)
  • For your References section:

    Role of tetanus neurotoxin insensitive vesicle-associated membrane protein (TI-VAMP) in vesicular transport mediating neurite outgrowth. Martinez-Arca S, Alberts P, Zahraoui A, Louvard D, Galli T. J Cell Biol. 2000 May 15;149(4):889-900. 10.1083/jcb.149.4.889 PubMed 10811829