Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42331||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerBD PharMingen
- Backbone size w/o insert (bp) 4661
Vector typeMammalian Expression
Growth in Bacteria
Alt namelate endosomal/lysosomal adaptor, MAPK and MTOR activator 1
SpeciesH. sapiens (human)
GenBank IDNM_017907.2 NP_060377.1
Entrez GeneLAMTOR1 (a.k.a. C11orf59, PDRO, Ragulator1, p18, p27RF-Rho)
- Promoter CMV, SP6
/ Fusion Protein
- Flag (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (destroyed during cloning)
- 5′ sequencing primer Sp6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRK5-p18-FLAG was a gift from David Sabatini (Addgene plasmid # 42331 ; http://n2t.net/addgene:42331 ; RRID:Addgene_42331)
For your References section:Ragulator Is a GEF for the Rag GTPases that Signal Amino Acid Levels to mTORC1. Bar-Peled L, Schweitzer LD, Zoncu R, Sabatini DM. Cell. 2012 Sep 14;150(6):1196-208. doi: 10.1016/j.cell.2012.07.032. 10.1016/j.cell.2012.07.032 PubMed 22980980