|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42418||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)Mach1 ;
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)406
/ Fusion Protein
- His6-TEV (N terminal on insert)
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
http://www.thesgc.org/structures/3EQ5/ Use BL21(DE3)-R3-pRARE2 strain for expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:SKILA was a gift from Nicola Burgess-Brown (Addgene plasmid # 42418 ; http://n2t.net/addgene:42418 ; RRID:Addgene_42418)