pEXP5-NT-7th R
(Plasmid #42573)

• Depositing Lab: Ivan Korendovych
• Publication: Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi: 10.1002/anie.201302339.

Backbone

• Vector backbone: pEXP5-NT/TOPO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 2745
• Total vector size (bp): 3194
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: mutated calmodulin gene
• Species: G. gallus (chicken)
• Insert Size (bp): 449
• Mutation: changed Phenylalanine 92 to Glutamine acid, Isoleucine 85 to Leucine, Alanine 88 to Glutamine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 124 to Leucine, Alanine 128 to Threonine, Methionine 144 to Arginine
• Promoter: T7
• Tag / Fusion Protein:
  • Polyhistidine (6×His) region, TEV recognition site (N terminal on insert)

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry