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p3s-Cas9HC
(Plasmid #43945)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43945 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 43945-D50 50 μg of DNA in Tris buffer $495
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DNA 43945-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCDNA3.1
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 3200
  • Total vector size (bp) 7400
  • Modifications to backbone
    PvuII digestion and re-ligation to delete non-essential element in p3-Cas9HC.
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL10 Gold
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4170
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • HA epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For more information on Kim Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/Kim/

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3s-Cas9HC was a gift from Jin-Soo Kim (Addgene plasmid # 43945 ; http://n2t.net/addgene:43945 ; RRID:Addgene_43945)

  • For your References section:

    Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease. Cho SW, Kim S, Kim JM, Kim JS. Nat Biotechnol. 2013 Jan 29. doi: 10.1038/nbt.2507. 10.1038/nbt.2507 PubMed 23360966
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