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pMJ920
(Plasmid #42234)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42234 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MacroLab 6D
  • Backbone manufacturer
    QB3 MacroLab
  • Backbone size w/o insert (bp) 6145
  • Total vector size (bp) 10357
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Mach1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Alt name
    Csn1
  • Species
    Synthetic; Streptococcus pyogenes
  • Insert Size (bp)
    4209
  • Mutation
    codon-optimized synthetic DNA sequence
  • GenBank ID
    NP_269215.1 NC_002737.1 (854757..858863)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA epitope (C terminal on insert)
    • NLS (nuclear localization signal) (C terminal on insert)
    • GFP (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer BGH polyA
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information on Doudna Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/doudna/

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMJ920 was a gift from Jennifer Doudna (Addgene plasmid # 42234 ; http://n2t.net/addgene:42234 ; RRID:Addgene_42234)
  • For your References section:

    RNA-programmed genome editing in human cells. Jinek M, East A, Cheng A, Lin S, Ma E, Doudna J. elife. 2013;2:e00471. doi: 10.7554/eLife.00471. Epub 2013 Jan 29. 10.7554/eLife.00471 PubMed 23386978