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pBMNZ-neo-Flag-TNFR1 L380A
(Plasmid #43949)

Full plasmid sequence is not available for this item.



Item Catalog # Description Quantity Price (USD)
Plasmid 43949 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Nolan Lab
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    Tumor Necrosis Factor Receptor 1
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
  • GenBank ID
  • Entrez Gene
    TNFRSF1A (a.k.a. CD120a, FPF, TBP1, TNF-R, TNF-R-I, TNF-R55, TNFAR, TNFR1, TNFR55, TNFR60, p55, p55-R, p60)
  • Tag / Fusion Protein
    • Flag* (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*The N-terminus not only has a Flag tag, but starting with methionine has a signal sequence that replaces the first 29 amino acids of the TNFR1 coding sequence: MGLSTVPDLLLPLVLLELLVGIYPSGVIG

Downstream of the Flag tag, the coding sequence of this construct is a 100% match with the reference sequence NM_001065 except for the desirable L380A mutation that is already noted for this entry.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBMNZ-neo-Flag-TNFR1 L380A was a gift from Martin Kluger (Addgene plasmid # 43949 ; ; RRID:Addgene_43949)
  • For your References section:

    Targeting of tumor necrosis factor receptor 1 to low density plasma membrane domains in human endothelial cells. D'Alessio A, Kluger MS, Li JH, Al-Lamki R, Bradley JR, Pober JS. J Biol Chem. 2010 Jul 30;285(31):23868-79. doi: 10.1074/jbc.M110.122853. Epub 2010 May 28. 10.1074/jbc.M110.122853 PubMed 20511226