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Depositing Lab
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Depositing OrganizationUniversity of Edinburgh
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 43961 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 43961-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 43961-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDONR221-P1P5r
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 2612
- Total vector size (bp) 5366
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Vector typeMultisite Gateway ENTR clone
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP-CreERT2
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Alt nameCreERT2-eGFP
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Alt nameGFP-CreERT2
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Insert Size (bp)2754
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byDr. Akio Kobayashi
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 43961-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 43961-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR eGFPCreERT2 L1-R5 was a gift from Peter Hohenstein (Addgene plasmid # 43961 ; http://n2t.net/addgene:43961 ; RRID:Addgene_43961) -
For your References section:
A universal vector for high-efficiency multi-fragment recombineering of BACs and knock-in constructs. Dolt KS, Lawrence ML, Miller-Hodges E, Slight J, Thornburn A, Devenney PS, Hohenstein P. PLoS One. 2013 Apr 29;8(4):e62054. doi: 10.1371/journal.pone.0062054. Print 2013. 10.1371/journal.pone.0062054 PubMed 23637962
Map uploaded by the depositor.
