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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44021 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCTcon2
- Backbone size w/o insert (bp) 6142
- Total vector size (bp) 7078
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHRP
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SpeciesSynthetic
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Insert Size (bp)936
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Tags
/ Fusion Proteins
- HA (N terminal on backbone)
- c-myc (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer pCT-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCT2-HRP was a gift from Dane Wittrup (Addgene plasmid # 44021 ; http://n2t.net/addgene:44021 ; RRID:Addgene_44021) -
For your References section:
Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. Lipovsek D, Antipov E, Armstrong KA, Olsen MJ, Klibanov AM, Tidor B, Wittrup KD. Chem Biol. 2007 Oct;14(10):1176-85. 10.1016/j.chembiol.2007.09.008 PubMed 17961829