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pRCIscript-crhr1_TAL1B
(Plasmid #44235)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44235 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    RCIscript-GoldyTALEN
  • Backbone manufacturer
    Addgene plasmid 38142
  • Backbone size w/o insert (bp) 4651
  • Total vector size (bp) 6657
  • Vector type
    mRNA transcription vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crhr1 pTAL scaffold 1B
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    crhr1
  • Promoter T3

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRCIscript-crhr1_TAL1B was a gift from Karl Clark & Stephen Ekker (Addgene plasmid # 44235 ; http://n2t.net/addgene:44235 ; RRID:Addgene_44235)
  • For your References section:

    In vivo genome editing using a high-efficiency TALEN system. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC. Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 2012 Sep 23. 10.1038/nature11537 PubMed 23000899