|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44235||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerAddgene plasmid 38142
- Backbone size w/o insert (bp) 4651
- Total vector size (bp) 6657
Vector typemRNA transcription vector
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecrhr1 pTAL scaffold 1B
SpeciesD. rerio (zebrafish)
- Promoter T3
- Cloning method Unknown
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
Please note that some of the NN RVDs of this TALEN plasmid, which recognize G nucleotides were actually made with NK RVDs (which also recognize G nucleotides). This alters a single amino acid within the RVD.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pRCIscript-crhr1_TAL1B was a gift from Karl Clark & Stephen Ekker (Addgene plasmid # 44235 ; http://n2t.net/addgene:44235 ; RRID:Addgene_44235)
For your References section:In vivo genome editing using a high-efficiency TALEN system. Bedell VM, Wang Y, Campbell JM, Poshusta TL, Starker CG, Krug RG 2nd, Tan W, Penheiter SG, Ma AC, Leung AY, Fahrenkrug SC, Carlson DF, Voytas DF, Clark KJ, Essner JJ, Ekker SC. Nature. 2012 Nov 1;491(7422):114-8. doi: 10.1038/nature11537. Epub 2012 Sep 23. 10.1038/nature11537 PubMed 23000899