Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44264||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 9800
Vector typeMammalian Expression, Mouse Targeting, Luciferase
Growth in Bacteria
Gene/Insert nameK15 promoter (-4.8)
Alt nameKrt1-15 promoter
Alt namemouse keratin 15 promoter
Alt nameKrt15 keratin 15
SpeciesM. musculus (mouse)
Insert Size (bp)4961
MutationContains the murine K15 promoter (-4.8) fragment
GenBank IDNC_000077.6 AF542050
Entrez GeneKrt15 (a.k.a. RP23-217I3.2, AI528832, K15, Krt1-15)
- Promoter murine K15 promoter (-4.8) fragment
/ Fusion Protein
- Luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer LucNRev; EBV-rev (Common Sequencing Primers)
Alternate plasmid name: K15(-4.8)-pGL3
The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were
respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGL3-Basic (Promega).
The entire K15 promoter-luciferase transgene can be released by digestion with XhoI/SalI.
For your Materials & Methods section:K15/pGL3 was a gift from George Cotsarelis (Addgene plasmid # 44264 ; http://n2t.net/addgene:44264 ; RRID:Addgene_44264)
For your References section:Keratin 15 promoter targets putative epithelial stem cells in the hair follicle bulge. Liu Y, Lyle S, Yang Z, Cotsarelis G. J Invest Dermatol. 2003 Nov;121(5):963-8. 10.1046/j.1523-1747.2003.12600.x PubMed 14708593
Map uploaded by the depositor.