Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||44267||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4818
- Total vector size (bp) 9800
Modifications to backboneLuciferase replaced with HSV-1 TK from a TK/PBSIIKS plasmid by NotI/SalI digestion
Vector typeMammalian Expression, Mouse Targeting ; Thymidine kinase
Selectable markersThymidine Kinase (HSV-1 TK)
Growth in Bacteria
Gene/Insert nameK15 promoter (-4.8)
Alt nameKrt1-15 promoter
Alt namemouse keratin 15 promoter
Alt nameKrt15 keratin 15
SpeciesM. musculus (mouse)
Insert Size (bp)4961
MutationContains the murine K15 promoter (-4.8) fragment
GenBank IDNC_000077.6 AF542050
Entrez GeneKrt15 (a.k.a. RP23-217I3.2, AI528832, K15, Krt1-15)
- Promoter murine K15 promoter (-4.8) fragment
/ Fusion Protein
- HSV-1 TK (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer RVprimer3
- 3′ sequencing primer pBR322ori-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Alternate plasmid name: K15-HSV-TK-pGL3
The K15 promoter was cloned from C57 BL/SJ mouse genomic DNA (isolated from tail) by PCR using the Supermix High Fidelity Kit (GIBCO). The sequences of the forward and reverse primers were
respectively. XhoI and EcoRI restriction sequences were added to the 5' end of forward and reverse primers respectively for subsequent cloning of the PCR product into PBK/CMV vector (Stratagene). The promoter fragment was then subcloned into pGL3-Basic (Promega) to create plasmid K15(-4.8)-pGL3 (Addgene plasmid #44264).
The HSV-1 TK reporter gene cDNA was then excised from a TK/PBSIIKS plasmid, using NotI and SalI, and cloned downstream of the K15 promoter, replacing luciferase.
The entire K15 promoter-HSV-TK transgene can be released by digestion with XhoI/SalI.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:K15-TK/pGL3 was a gift from George Cotsarelis (Addgene plasmid # 44267 ; http://n2t.net/addgene:44267 ; RRID:Addgene_44267)
For your References section:Stem cells in the hair follicle bulge contribute to wound repair but not to homeostasis of the epidermis. Ito M, Liu Y, Yang Z, Nguyen J, Liang F, Morris RJ, Cotsarelis G. Nat Med. 2005 Dec;11(12):1351-4. Epub 2005 Nov 20. 10.1038/nm1328 PubMed 16288281
Map uploaded by the depositor.