pMX-mTAF4b
(Plasmid #44345)

• Depositing Lab: Marc Timmers
• Publication: Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi: 10.1038/nature11970. Epub 2013 Mar 17.

Backbone

• Vector backbone: pMX
• Total vector size (bp): 7544
• Vector type: Mammalian Expression, Retroviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Standard growth
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TAF4b
• Alt name: TBP-associated factor 4b
• Species: M. musculus (mouse)
• Insert Size (bp): 2550
• GenBank ID: NM_001100449.1
• Entrez Gene: Taf4b (a.k.a. 105kDa, 2610524B04Rik, 4932409F03Rik, AW987595, TAFII-105, TAFII105, Taf2c2)
• Promoter: LTR
• Tag / Fusion Protein:
  • flag and SBP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Not1 (not destroyed)
• 3′ cloning site: Sal1 (not destroyed)
• 5′ sequencing primer: pBMN_5_primer
• 3′ sequencing primer: -

Resource Information

• Supplemental Documents:
  • mTAF4B-pMX

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is a 5aa deletion in the full plasmid seq compared to NM_001100449.1.