pLPC-Flag-TRF2(delta)iDDR
(Plasmid #44577)

• Depositing Lab: Eros Lazzerini Denchi
• Publication: Okamoto et al Nature. 2013 Feb 6. doi: 10.1038/nature11873.

Backbone

• Vector backbone: pLPC
• Total vector size (bp): 6943
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TRF2ΔiDDR
• Alt name: TRF2deltaiDDR
• Species: H. sapiens (human)
• Insert Size (bp): 1428
• Mutation: iDDR domain (aa 407-431) has been deleted
• GenBank ID: NM_005652.3 NP_005643.1
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pLPC-5
• 3′ sequencing primer: pLPC-3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

TRF2 construct with the iDDR region removed (aa 407-431 when numbered according to GenBank accession number NP_005643.1). Deleted protein sequence is: PKGKWNSSNGVEEKETWVEEDELFQ