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K18iresCreER-T2
(Plasmid #44580)

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 44580 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    keratin 18 gene
  • Species
    H. sapiens (human)
  • Entrez Gene
    KRT18 (a.k.a. CK-18, CYK18, K18)
  • Tag / Fusion Protein
    • IRES_CreER-T2

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NA (unknown if destroyed)
  • 3′ cloning site NA (unknown if destroyed)
  • 5′ sequencing primer Sp6
  • 3′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    CreER was provided by Dr. Pierre Chambon.
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Cre recombinase-mutant estrogen receptor fusion (CreERT2) in K18 gene-ires vector for tamoxifen-dependent Cre activity confined to epithelial tissues. This is the full length K18 gene with an IRES inserted downstream of sixth exon TGA and before the polyA signal.

Please note that the partial plasmid sequence available for this plasmid is missing the human ERT2 sequence. This has been indicated in the partial sequence by a string of 10 N's at bp#7941-7950 immediately after the Cre sequence. See Addgene's sequencing result with the Cre-F primer for more information about this region.

Human K18 gene from pGC1853 (Addgene #44619).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    K18iresCreER-T2 was a gift from Robert Oshima (Addgene plasmid # 44580 ; http://n2t.net/addgene:44580 ; RRID:Addgene_44580)
  • For your References section:

    Expression of conditional cre recombinase in epithelial tissues of transgenic mice. Wen F, Cecena G, Munoz-Ritchie V, Fuchs E, Chambon P, Oshima RG. Genesis. 2003 Feb;35(2):100-6. 10.1002/gene.10169 PubMed 12533792