pGC1853
(Plasmid
#44619)
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Depositing Lab
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Depositing OrganizationSanford Burnham Prebys Medical Discovery Institute
Located in United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44619 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 44619-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 44619-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGC
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namekeratin 18 gene
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Alt namek18
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Alt nameKrt18
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SpeciesH. sapiens (human)
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Entrez GeneKRT18 (a.k.a. CK-18, CYK18, K18)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Whole human K18 gene including flanking regions and six exons. Best expression of K18 in transgenic or transfection
DNA (Catalog # 44619-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 44619-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGC1853 was a gift from Robert Oshima (Addgene plasmid # 44619 ; http://n2t.net/addgene:44619 ; RRID:Addgene_44619) -
For your References section:
Cloning of the human keratin 18 gene and its expression in nonepithelial mouse cells. Kulesh DA, Oshima RG. Mol Cell Biol. 1988 Apr;8(4):1540-50. PubMed 2454392
Map uploaded by the depositor.
