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BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)
(Plasmid #101178)


Item Catalog # Description Quantity Price (USD)
Plasmid 101178 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Joung lab (Addgene Plasmid #43861)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Insert Size (bp)
  • Mutation
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (SV40) (C terminal on insert)
    • 3x FLAG (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9) was a gift from Jennifer Doudna & Keith Joung (Addgene plasmid # 101178 ; ; RRID:Addgene_101178)
  • For your References section:

    Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. 10.1038/nature24268 PubMed 28931002