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Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
Nature. 2017 Sep 20. doi: 10.1038/nature24268. PubMed Article

Plasmids from Article

ID Plasmid  
101176BPK3258 - human expression plasmid for eSpCas9(1.1)
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101177BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1
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101178BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)
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101179MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926A
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101180MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926A
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101181MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926A
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101182MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926A
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101183MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926A
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101184MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926A
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101185MMW2993 - human expression plasmid for SpCas9 Cluster 2
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101187MMW3645 - human expression plasmid for SpCas9 Cluster 3
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101188MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926A
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101189MMW3770 - human expression plasmid for SpCas9 Cluster 4
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101190MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926A
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101191BPK4387 - human expression plasmid for SpCas9 Cluster 5
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101192BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926A
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101199pSHS207 - Bacterial expression plasmid for WT SpCas9
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101200pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variant
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101201pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variant
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101202pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variant
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101203pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
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101204pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
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101205pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domain
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101206pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variant
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101207pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
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101208pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
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101209pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variant
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101210pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variant
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101211pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
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101212pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
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101213pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variant
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101214pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
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101215pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
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101217pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
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101218pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
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101220pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
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101229pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
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101230pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
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101231pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
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101232pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
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101233pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variant
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101234pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
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101235pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
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101236pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
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