Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

Enhanced proofreading governs CRISPR-Cas9 targeting accuracy.
Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA
Nature. 2017 Sep 20. doi: 10.1038/nature24268.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
101176BPK3258 - human expression plasmid for eSpCas9(1.1)Human expression plasmid for SpCas9 eSpCas9(1.1) variant: CMV-T7-hSpCas9-eSpCas9(1.1)(K848A, K1003A, R1060A)-NLS(SV40)-3xFLAG
Loading...
101177BPK3274 - human expression plasmid for eSpCas9(1.1)-HF1Human expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant: CMV-T7-hSpCas9-eSpCas9(1.1)-HF1(N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A)-NLS(SV40)-3xFLAG
Loading...
101178BPK4410 - human expression plasmid for SpCas9 Cluster 1 (HypaCas9)Human expression plasmid for SpCas9 Cluster 1 variant (HypaCas9): CMV-T7-hSpCas9-Cluster1(N692A, M694A, Q695A, H698A)-NLS(SV40)-3xFLAG
Loading...
101179MMW3709 - human expression plasmid for SpCas9 Cluster 1 + Q926AHuman expression plasmid for SpCas9 Cluster 1 + Q926A variant: CMV-T7-hSpCas9-Cluster1+Q926A(N692A, M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101180MMW3914 - human expression plasmid for SpCas9 Cluster 1 H698 + Q926AHuman expression plasmid for SpCas9 Cluster 1 H698 + Q926A variant: CMV-T7-hSpCas9-Cluster1H698+Q926A(N692A, M694A, Q695A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101181MMW3689 - human expression plasmid for SpCas9 Cluster 1 Q695 + Q926AHuman expression plasmid for SpCas9 Cluster 1 Q695 + Q926A variant: CMV-T7-hSpCas9-Cluster1Q695+Q926A(N692A, M694A, H698A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101182MMW3911 - human expression plasmid for SpCas9 Cluster 1 M694 + Q926AHuman expression plasmid for SpCas9 Cluster 1 M694 + Q926A variant: CMV-T7-hSpCas9-Cluster 1M694+Q926A(N692A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101183MMW3909 - human expression plasmid for SpCas9 Cluster 1 N692 + Q926AHuman expression plasmid for SpCas9 Cluster 1 N692 + Q926A variant: CMV-T7-hSpCas9-Cluster1N692+Q926A(M694A, Q695A, H698A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101184MMW3001 - human expression plasmid for SpCas9 Cluster 2 + Q926AHuman expression plasmid for SpCas9 Cluster 2 + Q926A variant: CMV-T7-hSpCas9-Cluster2+Q926A(G582A, V583A, E584A, D585A, N588A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101185MMW2993 - human expression plasmid for SpCas9 Cluster 2Human expression plasmid for SpCas9 Cluster 2 variant: CMV-T7-hSpCas9-Cluster2(G582A, V583A, E584A, D585A, N588A)-NLS(SV40)-3xFLAG
Loading...
101187MMW3645 - human expression plasmid for SpCas9 Cluster 3Human expression plasmid for SpCas9 Cluster 3 variant: CMV-T7-hSpCas9-Cluster3(T657A, G658A, W659A, R661A)-NLS(SV40)-3xFLAG
Loading...
101188MMW3629 - human expression plasmid for SpCas9 Cluster 3 + Q926AHuman expression plasmid for SpCas9 Cluster 3 + Q926A variant: CMV-T7-hSpCas9-Cluster3+Q926A(T657A, G658A, W659A, R661A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101189MMW3770 - human expression plasmid for SpCas9 Cluster 4Human expression plasmid for SpCas9 Cluster 4 variant: CMV-T7-hSpCas9-Cluster4(F491A, M495A, T496A, N497A)-NLS(SV40)-3xFLAG
Loading...
101190MMW3759 - human expression plasmid for SpCas9 Cluster 4 + Q926AHuman expression plasmid for SpCas9 Cluster 4 + Q926A variant: CMV-T7-hSpCas9-Cluster4+Q926A(F491A, M495A, T496A, N497A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101191BPK4387 - human expression plasmid for SpCas9 Cluster 5Human expression plasmid for SpCas9 Cluster 5 variant: CMV-T7-hSpCas9-Cluster5(K918A, V922A, R925A)-NLS(SV40)-3xFLAG
Loading...
101192BPK4393 - human expression plasmid for SpCas9 Cluster 5 + Q926AHuman expression plasmid for SpCas9 Cluster 5 + Q926A variant: CMV-T7-hSpCas9-Cluster5+Q926A(K918A, V922A, R925A, Q926A)-NLS(SV40)-3xFLAG
Loading...
101199pSHS207 - Bacterial expression plasmid for WT SpCas9Bacterial expression plasmid for WT SpCas9
Loading...
101200pJSC033 - Bacterial expression plasmid for SpCas9, REC2 FRET variantBacterial expression plasmid for SpCas9, REC2 FRET variant
Loading...
101201pJSC052 - Bacterial expression plasmid for SpCas9, REC3 FRET variantBacterial expression plasmid for SpCas9, REC3 FRET variant
Loading...
101202pSHS273 - Bacterial expression plasmid for SpCas9∆REC3 variantBacterial expression plasmid for SpCas9∆REC3 variant
Loading...
101203pJSC038 - Bacterial expression plasmid for SpCas9∆REC3, HNH FRET variantBacterial expression plasmid for SpCas9∆REC3, HNH FRET variant
Loading...
101204pJSC057 - Bacterial expression plasmid for SpCas9∆REC3, REC2 FRET variantBacterial expression plasmid for SpCas9∆REC3, REC2 FRET variant
Loading...
101205pSHS325 - Bacterial expression plasmid for SpCas9 REC3 domainBacterial expression plasmid for SpCas9 REC3 domain
Loading...
101206pJSC176 - Bacterial expression plasmid for SpCas9 + Q926A variantBacterial expression plasmid for SpCas9 + Q926A variant
Loading...
101207pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variantBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
Loading...
101208pJSC120 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variantBacterial expression plasmid for SpCas9 + N497A/R661A/Q695A, HNH FRET variant
Loading...
101209pJSC111 - Bacterial expression plasmid for SpCas9-HF1 variantBacterial expression plasmid for SpCas9-HF1 variant
Loading...
101210pJSC115 - Bacterial expression plasmid for SpCas9-HF1, HNH FRET variantBacterial expression plasmid for SpCas9-HF1, HNH FRET variant
Loading...
101211pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variantBacterial expression plasmid for SpCas9-HF1, REC2 FRET variant
Loading...
101212pJSC131 - Bacterial expression plasmid for SpCas9-HF1, REC3 FRET variantBacterial expression plasmid for SpCas9-HF1, REC3 FRET variant
Loading...
101213pJSC090 - Bacterial expression plasmid for SpCas9 + K855A variantBacterial expression plasmid for SpCas9 + K855A variant
Loading...
101214pJSC077 - Bacterial expression plasmid for SpCas9 + K855A, HNH FRET variantBacterial expression plasmid for SpCas9 + K855A, HNH FRET variant
Loading...
101215pJSC114 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1) variantBacterial expression plasmid for SpCas9 eSpCas9(1.1) variant
Loading...
101217pJSC270 - Bacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variantBacterial expression plasmid for SpCas9 eSpCas9(1.1)-HF1 variant
Loading...
101218pJSC173 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variantBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9) variant
Loading...
101220pJSC269 - Bacterial expression plasmid for SpCas9 Cluster 1 + Q926A variantBacterial expression plasmid for SpCas9 Cluster 1 + Q926A variant
Loading...
101229pJSC011 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variantBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), HNH FRET variant
Loading...
101230pJSC281 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variantBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC2 FRET variant
Loading...
101231pJSC282 - Bacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variantBacterial expression plasmid for SpCas9 Cluster 1 (HypaCas9), REC3 FRET variant
Loading...
101232pJSC197 - Bacterial expression plasmid for SpCas9 Cluster 2 + Q926A variantBacterial expression plasmid for SpCas9 Cluster 2 + Q926A variant
Loading...
101233pJSC228 - Bacterial expression plasmid for SpCas9 Cluster 2 variantBacterial expression plasmid for SpCas9 Cluster 2 variant
Loading...
101234pJSC239 - Bacterial expression plasmid for SpCas9 Cluster 3 + Q926A variantBacterial expression plasmid for SpCas9 Cluster 3 + Q926A variant
Loading...
101235pJSC236 - Bacterial expression plasmid for SpCas9 Cluster 4 + Q926A variantBacterial expression plasmid for SpCas9 Cluster 4 + Q926A variant
Loading...
101236pJSC272 - Bacterial expression plasmid for SpCas9 Cluster 5 + Q926A variantBacterial expression plasmid for SpCas9 Cluster 5 + Q926A variant
Loading...