pSHS207 - Bacterial expression plasmid for WT SpCas9
PurposeBacterial expression plasmid for WT SpCas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101199||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDoudna lab
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameStreptococcus pyogenes Cas9
- Promoter T7
/ Fusion Proteins
- 10x His (N terminal on backbone)
- MBP (N terminal on backbone)
- TEV site (N terminal on backbone)
- Cloning method Gibson Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSHS207 - Bacterial expression plasmid for WT SpCas9 was a gift from Jennifer Doudna & Keith Joung (Addgene plasmid # 101199 ; http://n2t.net/addgene:101199 ; RRID:Addgene_101199)
For your References section:Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. 10.1038/nature24268 PubMed 28931002