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pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant
(Plasmid #101207)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 101207 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpCas9 variant N497A/R661A/Q695A
  • Alt name
    Streptococcus pyogenes Cas9
  • Species
    Streptococcus pyogenes
  • Mutation
    N497A, R661A and Q695A
  • Promoter T7
  • Tags / Fusion Proteins
    • 10x His (N terminal on backbone)
    • MBP (N terminal on backbone)
    • TEV site (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2017/08/12/160036 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJSC119 - Bacterial expression plasmid for SpCas9 + N497A/R661A/Q695A variant was a gift from Jennifer Doudna & Keith Joung (Addgene plasmid # 101207 ; http://n2t.net/addgene:101207 ; RRID:Addgene_101207)
  • For your References section:

    Enhanced proofreading governs CRISPR-Cas9 targeting accuracy. Chen JS, Dagdas YS, Kleinstiver BP, Welch MM, Sousa AA, Harrington LB, Sternberg SH, Joung JK, Yildiz A, Doudna JA. Nature. 2017 Sep 20. doi: 10.1038/nature24268. 10.1038/nature24268 PubMed 28931002