pTC62.13-dmd-1, full ORF, splice form 4
(Plasmid
#44688)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44688 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepJC53.2
- Backbone size w/o insert (bp) 3240
- Total vector size (bp) 4350
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Vector typeT/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSmed-dmd-1, full ORF, spice form 4
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SpeciesSchmidtea mediterranea
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Insert Size (bp)1110
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GenBank IDKC736558
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Eam1105I (destroyed during cloning)
- 3′ cloning site Eam1105I (destroyed during cloning)
- 5′ sequencing primer T3
- 3′ sequencing primer Sp6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTC62.13-dmd-1, full ORF, splice form 4 was a gift from Phillip Newmark (Addgene plasmid # 44688 ; http://n2t.net/addgene:44688 ; RRID:Addgene_44688) -
For your References section:
A sex-specific transcription factor controls male identity in a simultaneous hermaphrodite. Chong T, Collins JJ 3rd, Brubacher JL, Zarkower D, Newmark PA. Nat Commun. 2013; 4:1814. doi: 10.1038/ncomms2811. 10.1038/ncomms2811 PubMed 23652002