Flag-p50(1-435)
(Plasmid #44747)

• Depositing Lab: Gourisankar Ghosh
• Publication: Savinova et al Mol Cell. 2009 Jun 12;34(5):591-602. doi: 10.1016/j.molcel.2009.04.033.

Backbone

• Vector backbone: pEYFP-C1
• Backbone manufacturer: Clontech
• Modifications to backbone: YFP replaced with FLAG.
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: p50
• Species: M. musculus (mouse)
• Mutation: AA 1-435
• Entrez Gene: Nfkb1 (a.k.a. NF-KB1, NF-kappaB, NF-kappaB1, p105, p50, p50/p105)
• Tag / Fusion Protein:
  • FLAG (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NA (unknown if destroyed)
• 3′ cloning site: NA (unknown if destroyed)
• 5′ sequencing primer: CMV Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's sequencing results identified a K352N mutation in the p50 insert sequence compared to GenBank reference sequence NP_032715.2.