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pET15b-At 14-3-3 iota
(Plasmid #44937)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 44937 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET15b
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 6500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    14-3-3 iota
  • Alt name
    GRF12
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    807
  • GenBank ID
    NM_102411.2
  • Entrez Gene
    GRF12 (a.k.a. AT1G26480, GF14 IOTA, T1K7.15, T1K7_15, general regulatory factor 12)
  • Promoter T7
  • Tag / Fusion Protein
    • His tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 terminator
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b-At 14-3-3 iota was a gift from Robert Ferl (Addgene plasmid # 44937 ; http://n2t.net/addgene:44937 ; RRID:Addgene_44937)
  • For your References section:

    Exposed loop domains of complexed 14-3-3 proteins contribute to structural diversity and functional specificity. Sehnke PC, Laughner B, Cardasis H, Powell D, Ferl RJ. Plant Physiol. 2006 Feb;140(2):647-60. Epub 2006 Jan 11. 10.1104/pp.105.073916 PubMed 16407442