Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45171||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer GFP-N (Common Sequencing Primers)
Terms and Licenses
Please note that Addgene's sequencing result identified a single nucleotide mismatch at bp# 653 when compared to GenBank ID AF336304. This silent mutation does not affect the protein sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:mH2A2-CT-GFP was a gift from Brian Chadwick & Hunt Willard (Addgene plasmid # 45171 ; http://n2t.net/addgene:45171 ; RRID:Addgene_45171)
For your References section:Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant. Chadwick BP, Willard HF. Hum Mol Genet. 2001 May 1;10(10):1101-13. 10.1093/hmg/10.10.1101 PubMed 11331621