IF-GFP-ATRX
(Plasmid #45444)

• Purpose: ATRX expression vector
• Depositing Lab: Michael Dyer
• Publication: Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.1186/2193-1801-3-222. eCollection 2014.

Backbone

• Vector backbone: pEGFP C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 12000
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: As ATRX is prone to insertion by IS10, you should sequence exon 8 and the entire sequence on occasion but especially after culturing in E. coli. A good primer for sequencing is GCCAACATGAACTCCAGTTATG.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATRX
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 7400
• Mutation: isoform 2, missing codon E124
• Entrez Gene: ATRX (a.k.a. JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX)
• Promoter: PCMV
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP N sequencing primer
• 3′ sequencing primer: EGFP C sequencing primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It was made from a combination of ATRX sequence cloned by David Picketts and a short sequence engineered by Genscript.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.