pLEX_970_puro_DEST_YFP
(Plasmid #45295)

• Depositing Lab: William Hahn
• Publication: Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead of print]

Backbone

• Vector backbone: pLEX_970
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: YFP

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: pLEX-fwd
• 3′ sequencing primer: pLEX-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The YFP gene cloned from the pEntry-YFP vector in Addgene.
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The YFP open reading frame was PCR amplified with BP flanked primers and adding an open ending to the YFP gene. The PCR product was ligated into pDONR223 with a Gateway BP reaction and subsequently cloned into pLEX970 with a Gateway LR reaction.